📘 EduIQ Life Science: Methods in Biology Printed Notes 🧬
Ace your CSIR UGC-NET Life Science preparation with EduIQ’s “Methods in Biology” Printed Notes in English Medium! ✅
This notes cover the following topic :-
- Isolation and purification of RNA , DNA (genomic and plasmid) and proteins, different separation methods.
- Analysis of RNA, DNA and proteins by one and two dimensional gel electrophoresis, Isoelectric focusing gels.
- Molecular cloning of DNA or RNA fragments in bacterial and eukaryotic systems.
- Expression of recombinant proteins using bacterial, animal and plant vectors.
- Isolation of specific nucleic acid sequences
- Generation of genomic and cDNA libraries in plasmid, phage, cosmid, BAC and YAC vectors.
- In vitro mutagenesis and deletion techniques, gene knock out in bacterial and eukaryotic organisms.
- Protein sequencing methods, detection of post translation modification of proteins.
- DNA sequencing methods, strategies for genome sequencing.
- Methods for analysis of gene expression at RNA and protein level, large scale expression, such as micro array based techniques
- Isolation, separation and analysis of carbohydrate and lipid molecules RFLP, RAPD and AFLP techniques
🔬 Covers essential techniques & methodologies
📖 Well-structured, easy-to-understand content
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